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x gal  (Gold Biotechnology Inc)


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    Structured Review

    Gold Biotechnology Inc x gal
    Tagging a nanobody to the N-terminus <t>of</t> <t>β-gal</t> does not affect its activity. (A) Colonies of dH5α bacteria transformed with a plasmid containing the coding sequence for β-gal with Nb16 fused to its N-terminus via a 4AA (GSHV) linker and plated on an LB/Kan plate with IPTG and <t>X-gal.</t> The plate was photographed 24 h after incubation at 37 °C for 16 h. (B), (C), and (D) are the same as (A), except that the bacteria were induced to express β-gal with Nb16 fused to its N-terminus via a longer flexible peptide (GSGASGSHV), a Strep-tag-containing peptide (GSWSHPQFEKHV), or β-gal with purification tags, respectively.
    X Gal, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 96/100, based on 304 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/x-gal/X-Gal/pmc12860646-45-27-31
    Average 96 stars, based on 304 article reviews
    x gal - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Usage of nanobody-beta-galactosidase fusion in immunoassays and its application in detecting a peanut allergen"

    Article Title: Usage of nanobody-beta-galactosidase fusion in immunoassays and its application in detecting a peanut allergen

    Journal: Food Chemistry: Molecular Sciences

    doi: 10.1016/j.fochms.2026.100357

    Tagging a nanobody to the N-terminus of β-gal does not affect its activity. (A) Colonies of dH5α bacteria transformed with a plasmid containing the coding sequence for β-gal with Nb16 fused to its N-terminus via a 4AA (GSHV) linker and plated on an LB/Kan plate with IPTG and X-gal. The plate was photographed 24 h after incubation at 37 °C for 16 h. (B), (C), and (D) are the same as (A), except that the bacteria were induced to express β-gal with Nb16 fused to its N-terminus via a longer flexible peptide (GSGASGSHV), a Strep-tag-containing peptide (GSWSHPQFEKHV), or β-gal with purification tags, respectively.
    Figure Legend Snippet: Tagging a nanobody to the N-terminus of β-gal does not affect its activity. (A) Colonies of dH5α bacteria transformed with a plasmid containing the coding sequence for β-gal with Nb16 fused to its N-terminus via a 4AA (GSHV) linker and plated on an LB/Kan plate with IPTG and X-gal. The plate was photographed 24 h after incubation at 37 °C for 16 h. (B), (C), and (D) are the same as (A), except that the bacteria were induced to express β-gal with Nb16 fused to its N-terminus via a longer flexible peptide (GSGASGSHV), a Strep-tag-containing peptide (GSWSHPQFEKHV), or β-gal with purification tags, respectively.

    Techniques Used: Activity Assay, Bacteria, Transformation Assay, Plasmid Preparation, Sequencing, Incubation, Strep-tag, Purification

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    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Transgenic Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
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    Electron Microscopy:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Cell Culture:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Immunofluorescence:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
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    Expressing:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
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    Labeling:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
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    Bioprocessing:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Generated:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
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    Mutagenesis:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
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    Functional Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Activation Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
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    Antibody Labeling:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Gel Extraction:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Subcloning:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Plasmid Preparation:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    DNA Synthesis:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
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    cDNA Library Assay:

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    Irradiation:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
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    RNA Extraction:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Extraction:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Enzymatic Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    DNA Sequencing:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Blocking Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Immunohistochemistry:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Enzyme-linked Immunosorbent Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Purification:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Imaging:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Recombinant:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Selection:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    MTT Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Infection:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Comparison:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Angiogenesis Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Two Hybrid Screening:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Reverse Transcription:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Homogenization:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Immunolabeling:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Chick Chorioallantoic Membrane Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Centrifugation:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Software:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Variant Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Transmission Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Molecular Cloning:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    CRISPR:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Electrophoresis:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Modification:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Positive Control:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Inverted Microscopy:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    TaqMan Copy Number Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Bacteria:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Gentle:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Protein Concentration:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Spectrophotometry:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    In Situ Hybridization:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Dissection:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Lambda DNA Preparation:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Strep-tag:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Virus:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    SYBR Green Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    β-Gal Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    De-Phosphorylation Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Protein Extraction:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Fluorescence:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Over Expression:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Microscopy:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    DNA Purification:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Transfection:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Y2H Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Saline:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    X-gal Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Activity Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    End Labeling:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Cloning:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Chromatography:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Random Hexamer Labeling:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Polymerase Chain Reaction:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Enzyme-linked Immunospot:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Reporter Gene Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    High Performance Liquid Chromatography:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Magnetic Beads:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Titer Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Electroporation:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Colony Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Bradford Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Marker:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Synthesized:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Produced:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Knock-Out:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Construct:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    In Silico:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    In Vitro:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Neutralization:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Residue:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Agarose Gel Electrophoresis:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Amplification:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Injection:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Gradient Centrifugation:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Library Screening:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    RNAscope:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    TA Cloning:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Confocal Microscopy:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    TUNEL Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Hood:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Multiplex Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Pulsed-Field Gel:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    In Situ:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    DNA Extraction:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Cell Cycle Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Incubation:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Mouse Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Ligation:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Gel Purification:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Histopathology:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Cytotoxicity Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Molecular Weight:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Western Blot:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Dominant Negative Mutation:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Staining:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Flow Cytometry:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Immunohistochemical staining:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Retroviral:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Binding Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Biomarker Discovery:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Microarray:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Methylation:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Transformation Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Derivative Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    PCR Cloning:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Excision Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Immunostaining:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Bradford Protein Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Protease Inhibitor:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Real-time Polymerase Chain Reaction:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Northern Blot:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Hybridization:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Sterility:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Clone Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Concentration Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Immunocytochemistry:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Avidin-Biotin Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Isolation:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Fractionation:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Control:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Membrane:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Conjugation Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Genotyping Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Immunoperoxidase Staining:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Light Microscopy:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Knock-In:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Alkaline Lysis:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Luciferase:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Glycoproteomics:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Lysis:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Transduction:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    In Vivo:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Sequencing:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Invasion Assay:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks

    Negative Control:

    Article Title: Molecular genetic characterization of bacterial KH-domain proteins
    Article Snippet: PrePrint: ng reagents were purchased from New England Biolabs. Reagents specific to B2H/B3H assays were purchased from Novagen/Millipore Sigma (PopCulture Reagent, cat. no. 71092; rLysozyme, cat. no. 71110) and Gold Biotechnology (X-gal, cat. no. X4281; TPEG (phenylethyl-β-D-thiogalactopyranoside), cat. no. P-125). Materials for 96-well bacterial growth and lysis were purchased from VWR (2mL deep well blocks



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    Figure Lengend Snippet: Tagging a nanobody to the N-terminus of β-gal does not affect its activity. (A) Colonies of dH5α bacteria transformed with a plasmid containing the coding sequence for β-gal with Nb16 fused to its N-terminus via a 4AA (GSHV) linker and plated on an LB/Kan plate with IPTG and X-gal. The plate was photographed 24 h after incubation at 37 °C for 16 h. (B), (C), and (D) are the same as (A), except that the bacteria were induced to express β-gal with Nb16 fused to its N-terminus via a longer flexible peptide (GSGASGSHV), a Strep-tag-containing peptide (GSWSHPQFEKHV), or β-gal with purification tags, respectively.

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